Môi trường nuôi cấy tế bào dùng cho nghiên cứu DMEM High Glucose w/ L-Glutamine w/o Sodium Bicarbonate w/ Sodium Pyruvate – For 5L

Môi trường nuôi cấy tế bào dùng cho nghiên cứu Môi trường nuôi cấy tế bào dùng cho nghiên cứu DMEM High Glucose w/ L-Glutamine w/o Sodium Bicarbonate w/ Sodium Pyruvate – For 5L

Dulbecco’s Modified Eagle’s Medium
High Glucose w/L-Glutamine w/Sodium Pyruvate w/o Sodium Bicarbonate
CAT N° : P0102
Theoretical pH : 6.3 ± 0.3
Osmolality : 255 mOsm/kg ± 10%
Colour : yellow -orange powder , orange in liquid
Storage conditions : +2°C to +8°C
Shelf life : 36 months
Endotoxin : < 1 EU/ml
Composition : Displayed on website and in catalogue; also available on request.
Recommended use :
– Respect storage conditions of the product
– Do not use the product after its expiry date
– Store the product in a dry area
– Wear clothes adapted to the manipulation of the product to avoid contamination (e.g. : gloves, mask,
hygiene cap, overall…)
– Protect the product from any form of humidity
– Use, in one time, after opening, the entire quantity of product of the container, without making a
concentrated solution (to avoid the formation of precipitates). If it is not possible, close the container
immediately after sampling the quantity of powder required.
– Supplements can be added prior to sterile filtration of the medium or aseptically introduced to sterile
medium (respect the final concentration of the media). The nature of the supplements may affect
storage conditions and shelf life of the medium.
The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or
veterinary applications.
Preparation instructions :
1) Measure out 90% of final required volume of water. Water temperature should be 15-20°C.
2) While gently stirring the water, add the powdered medium (13.535 g/litre). Stir until dissolved. Do
not heat.
3) Rinse original package with a small amount of water to remove all traces of powder. Add to
solution in step 2.
4) To the solution in step 3, add 3.7g of sodium bicarbonate (CAT N° : P2060) or 49.3ml of sodium
bicarbonate solution (7.5% w/v) (CAT N° : L0680) for each litre of final volume of medium being
prepared. Stir until dissolved.
5) While stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH since it may
rise during filtration. The use of 1 N HCl or 1 N NaOH is recommended.
6) Add additional water to bring the solution to final volume.
7) Sterilize immediately by filtration using a membrane with a porosity of 0.22 microns.
8) Aseptically dispense medium into sterile container.
Indications of deterioration :
Dry powder medium should be free flowing. Do not use if powder caked. Prepared medium should be
cleared of particulates and flocculent material. Do not use if liquid medium is cloudy or contains
precipitate. Other evidence of deterioration may include colour change or degradation of physical or
performance characteristics.

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